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KMID : 0603820070130040319
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Journal of Experimental & Biomedical Science
2007 Volume.13 No. 4 p.319 ~ p.324
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Improved Detection of Mycobacterium leprae by One-tube Nested Polymerase Chain Reaction
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Wang Hye-Young
Whang Joo-Hwan
Kim Jong-Pill
Cho Jang-Eun
Bang Hye-Eun
Lee Hye-Young
Cho Sang-Nae
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Abstract
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One-tube nested polymerase chain reaction (PCR) was evaluated for its efficacy in detecting Mycobacterium leprae in biopsy samples from leprosy patients. Primers were derived from the M leprae-specific element (RLEP) sequences which yield a 230 bp fragment. The specificity and the sensitivity of the one-tube nested PCR were compared with those of single PCR for detecting M leprae. The results showed that the one-tube nested PCR was about 100 times more sensitive than that of the single indicating the one-tube nested primer sets developed in this study can be an effective screening tool for the detection of M leprae in clinical diagnostic laboratories.
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KEYWORD
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Mycobacterium leprae, Repetitive element sequence (RLEP), One-tube nested PCR
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